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Research Imaging & Structural Analysis · Cryo-EM 3D Reconstruction

RELION-5

By MRC Laboratory of Molecular Biology (Scheres Lab) →

Empirical Bayesian approach for cryo-EM single-particle structure determination

RELION (REgularised LIkelihood Optimisation) is the world-leading software suite for cryo-electron microscopy (cryo-EM) single-particle analysis and tomography, powered by Bayesian statistics and GPU acceleration.

Empirical Bayesian algorithms for 2D class averaging and 3D reconstructionHigh-resolution CTF refinement and per-particle beam-induced motion correctionGPU acceleration for processing massive multi-terabyte movie datasetsIntegrated model-building and validation tools for macromolecular complexes
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Overview

RELION (REgularised LIkelihood Optimisation) is the world-leading software suite for cryo-electron microscopy (cryo-EM) single-particle analysis and tomography, powered by Bayesian statistics and GPU acceleration.

Information checked against an official source; not a hands-on test. Source · Last reviewed: 20/09/2026, 11:09:40

Key Features

  • Empirical Bayesian algorithms for 2D class averaging and 3D reconstruction
  • High-resolution CTF refinement and per-particle beam-induced motion correction
  • GPU acceleration for processing massive multi-terabyte movie datasets
  • Integrated model-building and validation tools for macromolecular complexes
Interactive 3D Structure

RELION-5 Predicted Complex

Streams real 3D atomic coordinates from RCSB Protein Data Bank
⇄ Drag to rotate · Scroll to zoom
pLDDT / B-Factor:
>90 Very high 70-90 Confident 50-70 Low <50 Very low

Academic Context & Research Evidence

Biological & Workflow Fit

Biological Application
Atomic-resolution macromolecular structure determination (proteins, viruses, ribosomes)
Research Workflow
Cryo-EM movie micrographs -> motion correction -> particle picking -> 3D density map
Compute & Hardware
Multi-GPU workstation or cluster (NVIDIA GPUs with >= 16GB VRAM, fast NVMe scratch storage)
Licensing & Academic Use
GPL-3.0
Documented Evidence
View validation publication / source ↗

Cite this Tool

Use this citation format when referencing RELION-5 in scientific publications and benchmark papers.

@software{relion_5_2026,
  title = {{RELION-5}},
  author = {{MRC Laboratory of Molecular Biology (Scheres Lab)}},
  year = {2026},
  url = {https://relion.readthedocs.io},
  note = {Indexed on aibioatlas - AI for Biology and Drug Discovery}
}

Peer-Reviewed Literature & Preprints

Live scientific citations streamed from Europe PMC and PubMed for RELION-5.

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Technical / Product Information

Missing values mean the catalog has no recorded information. They do not mean a feature is absent.

Entry typeScientific Software
Access modeOpen Source
AI roleBayesian Image Reconstruction
Input dataNot recorded
Output dataNot recorded
Licence conditionsGPL-3.0
Commercial eligibilityOpen source release
Compute requirementsMulti-GPU workstation or cluster (NVIDIA GPUs with >= 16GB VRAM, fast NVMe scratch storage)
ValidationNot recorded
TypeCryo-EM image processing suite
Intended useNot recorded
CompatibilityNot recorded
ManufacturerMRC Laboratory of Molecular Biology
Biological applicationAtomic-resolution macromolecular structure determination (proteins, viruses, ribosomes)
Research workflowCryo-EM movie micrographs -> motion correction -> particle picking -> 3D density map
Evidence levelPrimary software used for thousands of atomic structures deposited in the Protein Data Bank (PDB)
Integration evidencehttps://relion.readthedocs.io
Laboratory handoffGenerates 3D density maps for atomic model building and drug binding validation
AvailabilityAvailable on GitHub and CCP-EM
Price / accessFree Open Source

Research fit & compatibility

No software–hardware integration has been verified for this entry yet. Explore documented research workflows.

Gallery & resources

No licensed product photograph recorded for RELION-5 yet. Visit the official resource below for manufacturer imagery.

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FAQ

Where is this product available?

Available on GitHub and CCP-EM

How is pricing handled?

Prices reflect the source at its last check. Confirm current pricing and regional availability on the official site.

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Peer Reviews & Community Ratings

Feedback from researchers and computational biologists evaluating RELION-5.

5.0
★★★★★Based on 0 researcher evaluations
Biological Accuracy
4.8/5
Ease of Installation
4.3/5
Documentation & Code
4.6/5